Patent Portfolio
Method for identifying intracellular endogenous protein
Ref. No. | AJ144 |
---|---|
Appl. No. (filing date) |
PCT/JP2016/085879(2016/12/2) |
Pub. No. (Pub. date) |
WO2017/094878(2017/6/8) |
Priority claim No. (Priority date) |
JP2015-236040 (2015/12/2) |
Patent No. | - |
Inventor | SAITO Hirohide, etc. |
Assignee | Kyoto University |
Title of Invention | Method for identifying intracellular endogenous protein |
Abstract | Provided is a method for detecting an intracellular endogenous protein in living cells comprising steps of (1) introducing into a cell, an intracellular endogenous protein-responsive mRNA that contains an aptamer sequence unique to the protein and a marker gene sequence functionally linked thereto, and (2) identifying the intracellular endogenous protein on the basis of the amount of translation of the marker gene. Also provided are a method for detecting a cell that expresses the intracellular endogenous protein, and a stabilized aptamer unique to an intracellular endogenous protein, for example, LIN28. |
Comments |
- |
Updated date | 2017/9/5 |
Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2017-094878/A6C5C50682A844D400AF3FBB8819E386D60D944A898C5023CEA961887F96B851/50/en |