Patent Portfolio

Method for identifying intracellular endogenous protein

Ref. No. AJ144
Appl. No.
(filing date)
PCT/JP2016/085879(2016/12/2)
Pub. No.
(Pub. date)
WO2017/094878(2017/6/8)
Priority claim No.
(Priority date)

JP2015-236040 (2015/12/2)

Patent No.

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Inventor SAITO Hirohide, etc.
Assignee Kyoto University
Title of Invention Method for identifying intracellular endogenous protein
Abstract

Provided is a method for detecting an intracellular endogenous protein in living cells comprising steps of (1) introducing into a cell, an intracellular endogenous protein-responsive mRNA that contains an aptamer sequence unique to the protein and a marker gene sequence functionally linked thereto, and (2) identifying the intracellular endogenous protein on the basis of the amount of translation of the marker gene. Also provided are a method for detecting a cell that expresses the intracellular endogenous protein, and a stabilized aptamer unique to an intracellular endogenous protein, for example, LIN28.

Comments

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Updated date 2017/9/5
Information
(External link)
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2017-094878/A6C5C50682A844D400AF3FBB8819E386D60D944A898C5023CEA961887F96B851/50/en