Related Patent Info

METHOD FOR PREPARATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE THROUGH CELL DIFFERENTIATION AND MATURATION AND HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE PREPARED BY SAME METHOD

Inventor WOO, Donghun, etc.
Assignee NEXEL CO.,LTD.
Title of Invention METHOD FOR PREPARATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE THROUGH CELL DIFFERENTIATION AND MATURATION AND HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE PREPARED BY SAME METHOD
Abstract

Provided is a method for preparing human pluripotent stem cell (hPSC)-derived functional cardiomyocytes through cell differentiation and maturation, comprising steps of (i) culturing hPSC in a Matrigel-coated culture medium, (ii) inducing the differentiation of hPSC in step (i) to mesoderm in a mesodermal induction medium containing insulin-free B27 and a wnt signaling activator, (iii) differentiating the cells in step (ii) in a differentiation medium containing insulin-free B27, (iv) inducing myocardial differentiation of the cells in step (iii) in a myocardial differentiation inducing medium containing insulin-free B27 and a wnt signaling regulator, and (v) maturing the differentiated cardiomyocytes in step (iv) into functional cardiomyocytes by culturing in a myocardial maturation medium containing vitamin A-free B27, IL-15, and NGF.