Related Patent Info

Provided is a method for producing an integrated human forebrain structure in vitro, comprising steps of inducing a neural fate in a pluripotent stem cell (e.g. an iPS cell) suspension culture to provide a spheroid of neural progenitor cells, then (i) differentiating the neural progenitor cells in a spheroid into cortical spheroids (hCS) and (ii) differentiating the neural progenitor cells in a spheroid into subpallial spheroids (hSS) as well, and culturing the hCS and hSS under conditions permissive for cell fusion in neural medium in the absence of growth factors; wherein an integrated forebrain structure is produced comprising interacting GABAergic and glutamatergic neurons.