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Provided is a method for generating and isolating cardiomyocytes derived from pluripotent or multipotent stem cells, comprising steps of (a) differentiating said stem cells into cardiovascular cells, thereby generating a sample comprising cardiomyocytes and non-cardiomyocytes, (b) labeling the non-cardiomyocytes of said sample with more than one antibody or antigen binding fragment thereof specific for antigens of non-cardiomyocytes, wherein said more than one antibody or antigen-binding fragment thereof is selected from the group consisting of antiCDIO, antiCD105, antiCD140b, antiCD61, antiCD44, antiCD141, and antiCD51/61, and (c) depleting said labeled non-cardiomyocytes from said sample.