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METHODS FOR THE IN VITRO MANUFACTURE OF GASTRIC FUNDUS TISSUE AND COMPOSITIONS RELATED TO SAME

Inventor WELLS, James, etc.
Assignee CHILDREN'S HOSPITAL MEDICAL CENTER
Title of Invention METHODS FOR THE IN VITRO MANUFACTURE OF GASTRIC FUNDUS TISSUE AND COMPOSITIONS RELATED TO SAME
Abstract

Provided is an in vitro method for inducing a gastric fundus tissue comprising steps of (a) contacting a mammalian definitive endoderm cell, which may be derived from an iPS cell, with a wnt activator, a FGF activator, a BMP inhibitor, and retinoic acid to form a 3D posterior foregut spheroid, (b) suspending said 3D posterior foregut spheroid in a basement membrane matrix with a growth factor, a wnt activator, a EGF activator, a BMP inhibitor, and retinoic acid to induce a fundic lineage comprising fundic-type gastric organoids (hFGOs), (c) culturing said hFGOs with a wnt activator and a EGF activator, then with FGF 10, and (d) contacting the cultured hFGOs with a MEK inhibitor to form gastric fundus tissue comprising a functional fundic cell.