Related Patent Info

IN VIVO METHOD FOR DIFFERENTIATING HUMAN PLURIPOTENT STEM CELLS INTO ATRIAL CARDIOMYOCYTES

Inventor BRAAM, Stefan Robbert, etc.
Assignee PLURIOMICS B.V.
Title of Invention IN VIVO METHOD FOR DIFFERENTIATING HUMAN PLURIPOTENT STEM CELLS INTO ATRIAL CARDIOMYOCYTES
Abstract

Provided is an in vitro method for differentiating human pluripotent stem cells into atrial cardiomyocytes, comprising steps of (a) contacting the pluripotent stem cells with a Wnt-signaling agonist and/or a BMP-signaling agonist for a period of about 6 - 54 hours, and (b) contacting the contacted cells in step (a) with a Wnt-signaling antagonist for a period of about 6 - 54 hours, wherein the cells are contacted with a retinoic acid-signaling agonist within the first 96 hours of steps (a) and (b) for a period of at least 6 hours.