Related Patent Info
MICROGLIA DERIVED FROM PLURIPOTENT STEM CELLS AND METHODS OF MAKING AND USING THE SAME
| Inventor | DOUVARAS, Panagiotis, etc. |
|---|---|
| Assignee | NEW YORK STEM CELL FOUNDATION, INC. |
| Title of Invention | MICROGLIA DERIVED FROM PLURIPOTENT STEM CELLS AND METHODS OF MAKING AND USING THE SAME |
| Abstract | Provided is a method for generating microglial cells from human pluripotent stem cells, comprising steps of (a) culturing the pluripotent stem cells under conditions that induce myeloid differentiation, leading to the generation of CD14+ and/or CX3CR1+ microglial progenitor cells, and (b) culturing the CD14+ and/or CX3CR1+ microglial progenitor cells in either (i) a first microglial differentiation medium comprising IL-34, or (ii) a second microglial differentiation medium comprising M-CSF. |