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Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same Issued

Ref. No. AJ089
Appl. No. (filing date) PCT/IL2003/001030 (2003/12/7)
Pub. No. (Pub. date) WO2004/055155 (2004/7/1)
Priority claim No. (Priority date)

US 60/433,619 (2002/12/16)

Patent No.

JP4613069

Inventor AMIT Michal, etc.
Assignee Technion Research & Development Foundation Ltd.
Title of Invention Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
Abstract

Provided is a method of establishing a feeder cells-free and xeno-free human embryonic stem cell line capable of being maintained in an undifferentiated, pluripotent and proliferative state comprising steps of culturing human embryonic stem cells under culturing conditions devoid of feeder cells and including a matrix and a tissue culture medium supplemented with TGFβ1, bFGF and/or LIF.

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License available only in Japan.

Updated date 2016/5/12
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