Cell production method
Ref. No. | AJ228 |
---|---|
Appl. No. (filing date) | PCT/JP2019/042423 (2019/10/29) |
Pub. No. (Pub. date) | WO2020/090836 (2020/05/07) |
Priority claim No. (Priority date) | JP2018-204358 (2018/10/30) |
Patent No. | - |
Inventor | SAKURAI Hidetoshi, etc. |
Assignee | Kyoto University |
Title of Invention | Cell production method |
Abstract | Provided is a method for producing skeletal muscle cells from pluripotent stem cells comprising steps of: (1) culturing the pluripotent stem cells under a condition for expressing one or more exogenous factors selected from MyoD and Myf5; (2) culturing the cells obtained in step (1) under a condition for not expressing the exogenous factors; and (3) culturing the cells obtained in step (2) under a condition for expressing one or more exogenous factors selected from MyoD and Myf5. The skeletal muscle cells obtained by the invention can be subjected to long-term culture for at least two weeks and are of high maturity. |
Comments | - |
Updated date | 2020/11/11 |
Publication |