Cell production method
|Appl. No. (filing date)||PCT/JP2019/042423 (2019/10/29)|
|Pub. No. (Pub. date)||WO2020/090836 (2020/05/07)|
|Priority claim No. (Priority date)||
|Inventor||SAKURAI Hidetoshi, etc.|
|Title of Invention||Cell production method|
Provided is a method for producing skeletal muscle cells from pluripotent stem cells comprising steps of: (1) culturing the pluripotent stem cells under a condition for expressing one or more exogenous factors selected from MyoD and Myf5; (2) culturing the cells obtained in step (1) under a condition for not expressing the exogenous factors; and (3) culturing the cells obtained in step (2) under a condition for expressing one or more exogenous factors selected from MyoD and Myf5. The skeletal muscle cells obtained by the invention can be subjected to long-term culture for at least two weeks and are of high maturity.