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Cell production method

Ref. No. AJ228
Appl. No. (filing date) PCT/JP2019/042423 (2019/10/29)
Pub. No. (Pub. date) WO2020/090836 (2020/05/07)
Priority claim No. (Priority date)

JP2018-204358 (2018/10/30)

Patent No.

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Inventor SAKURAI Hidetoshi, etc.
Assignee Kyoto University
Title of Invention Cell production method
Abstract

Provided is a method for producing skeletal muscle cells from pluripotent stem cells comprising steps of: (1) culturing the pluripotent stem cells under a condition for expressing one or more exogenous factors selected from MyoD and Myf5; (2) culturing the cells obtained in step (1) under a condition for not expressing the exogenous factors; and (3) culturing the cells obtained in step (2) under a condition for expressing one or more exogenous factors selected from MyoD and Myf5. The skeletal muscle cells obtained by the invention can be subjected to long-term culture for at least two weeks and are of high maturity.

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Updated date 2020/11/11
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